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rat collagen i alpha 1  (Novus Biologicals)


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    Novus Biologicals rat collagen i alpha 1
    Figure 5. Modulation of key paracrine factors involved in pathways of interest in the human secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet- derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I <t>alpha</t> 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.
    Rat Collagen I Alpha 1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+collagen+i+alpha+1/pm39996773-130-15-20?v=Novus+Biologicals
    Average 93 stars, based on 2 article reviews
    rat collagen i alpha 1 - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Secretome Analysis of Human and Rat Pancreatic Islets Co-Cultured with Adipose-Derived Stromal Cells Reveals a Signature with Enhanced Regenerative Capacities."

    Article Title: Secretome Analysis of Human and Rat Pancreatic Islets Co-Cultured with Adipose-Derived Stromal Cells Reveals a Signature with Enhanced Regenerative Capacities.

    Journal: Cells

    doi: 10.3390/cells14040302

    Figure 5. Modulation of key paracrine factors involved in pathways of interest in the human secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet- derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.
    Figure Legend Snippet: Figure 5. Modulation of key paracrine factors involved in pathways of interest in the human secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet- derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.

    Techniques Used: Derivative Assay, Comparison, Co-Culture Assay

    Figure 6. Modulation of key paracrine factors involved in pathways of interest in the rat secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet-derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.
    Figure Legend Snippet: Figure 6. Modulation of key paracrine factors involved in pathways of interest in the rat secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet-derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.

    Techniques Used: Derivative Assay, Comparison, Co-Culture Assay



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    Figure 5. Modulation of key paracrine factors involved in pathways of interest in the human secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet- derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I <t>alpha</t> 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.
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    Image Search Results


    Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 (Col1A1) ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).

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    Article Title: C57BL/6J mice best recapitulate fibrosis and inflammatory pathophysiology in syngeneic mouse model of endometriosis

    doi: 10.1038/s41598-025-13900-9

    Figure Lengend Snippet: Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 (Col1A1) ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).

    Article Snippet: The levels of Collagen Type I (COL1A1) in ectopic tissue lysates were quantified using a commercially available ELISA kit (Krishgen Biosystems, USA, #111111111).

    Techniques: Staining, Enzyme-linked Immunosorbent Assay

    Figure 5. Modulation of key paracrine factors involved in pathways of interest in the human secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet- derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.

    Journal: Cells

    Article Title: Secretome Analysis of Human and Rat Pancreatic Islets Co-Cultured with Adipose-Derived Stromal Cells Reveals a Signature with Enhanced Regenerative Capacities.

    doi: 10.3390/cells14040302

    Figure Lengend Snippet: Figure 5. Modulation of key paracrine factors involved in pathways of interest in the human secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet- derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.

    Article Snippet: Levels of rat and human bFGF, PDGF, VEGF (DuoSet ELISA; R&D Systems, Abingdon, UK), and rat collagen I alpha 1 (Novus Biologicals, Biotechne, Minneapolis, USA) were quantified through ELISA.

    Techniques: Derivative Assay, Comparison, Co-Culture Assay

    Figure 6. Modulation of key paracrine factors involved in pathways of interest in the rat secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet-derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.

    Journal: Cells

    Article Title: Secretome Analysis of Human and Rat Pancreatic Islets Co-Cultured with Adipose-Derived Stromal Cells Reveals a Signature with Enhanced Regenerative Capacities.

    doi: 10.3390/cells14040302

    Figure Lengend Snippet: Figure 6. Modulation of key paracrine factors involved in pathways of interest in the rat secretome upon co-culturing. Secretion of (A) vascular endothelial growth factor (VEGF), (B) platelet-derived growth factor (PDGF), (C) basic fibroblast growth factor (bFGF), (D) collagen I alpha 1, (E) interleukin 1 alpha (IL-1α), and (F) IL-10 after 72 h co-culturing under different conditions. Data represent mean values ± standard deviations of 3 pooled samples measured in triplicate. Two-way ANOVA (TWA) followed by Šídák’s multiple comparison test; * p < 0.05 versus normoxia islets alone; # p < 0.05 versus normoxia co-culture (1:1000); $ p < 0.05 versus respective condition islets alone.

    Article Snippet: Levels of rat and human bFGF, PDGF, VEGF (DuoSet ELISA; R&D Systems, Abingdon, UK), and rat collagen I alpha 1 (Novus Biologicals, Biotechne, Minneapolis, USA) were quantified through ELISA.

    Techniques: Derivative Assay, Comparison, Co-Culture Assay